T7
T7rnap

Part:BBa_I716103

Designed by: David Tulga   Group: iGEM07_Berkeley_UC   (2007-06-09)

T7rnap (T7 RNA Polymerase) - ATG Start Codon

This is the T7 RNA Polymerase gene (ATG Start), and will transcribe any genes with a T7 Promoter. It provides very good PoPS (Polymerases Per Second) output, and it is highly orthogonal to E. Coli's natural transcription system. This variation has an ATG start, and so is more active than the GTG start variation, but is also significantly toxic and potentially unstable in a -80 stock.

For further information, [http://2007.igem.org/BerkiGEM2007Present3 see here].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None